Fig. 1

Multiple sequence alignment of Psoroptes ovis var. cuniculi serpin genes Pso c 27 (a) and PsoSP2 (b). a Pso c 27. Multiple sequence alignment of the deduced amino acid sequence of Pso c 27 with homologous sequences of related proteins of other parasites: P. ovis (PSOVI22g04610), Dermatophagoides farinae (GenBank: AIO08851.1), D. pteronyssinus (GenBank: ATI08940.1), Euroglyphus maynei (GenBank: OTF72764.1), and Sarcoptes scabiei (GenBank: AEB40052.1). b PsoSP2. Multiple sequence alignment of the deduced amino acid sequence of PsoSP2 with homologous sequences of related proteins of other parasites: P. ovis (PSOVI22g04560), Euroglyphus maynei (GenBank: OTF74296.1), Dermatophagoides pteronyssinus (GenBank: XP_027200949.1), Sarcoptes scabiei (GenBank: KPM10873.1), and Dermatophagoides farinae (GenBank: AAP35082.1). Psoroptes ovis (PSOVI22g04610) and P. ovis (PSOVI22g04560) were obtained from the Online Resource for Community Annotation of Eukaryotes (OrcAE) (https://bioinformatics.psb.ugent.be/orcae/overview/Psovi). Helices are indicated with red horizontal bars. Horizonatal dark-green arrows along the sequence indicate sheets. Elements of secondary structure are labeled as: hA, hB, etc. A-helix, B-helix, etc., s1A, s2A, etc. strand 1 of the A β-sheet, strand 2 of the A β-sheet, etc. Consistent residues are highlighted with a dark-blue background, and consistent partial residues are highlighted with a light-blue background. B-cell epitopes are marked with a black box