Fig. 3

Immunoblotting with the serum-binding recombinant Pso c 27 and PsoSP2. Lanes M Protein molecular weight marker, 1 recombinant proteins of Escherichia coli expressing pET32a(+)-Pso c 27 induced by the reagent IPTG, 2 recombinant proteins of E. coli expressing pET32a(+)-PsoSP2 produced by IPTG, 3 proteins of E. coli expressing pET32a(+), 4 purified rPso c 27, 5 purified rPsoSP2, 6 purified rPso c 27 immunoblotted with P. ovis var. cuniculi-positive serum from rabbits with psoroptic mange, 7 purified rPsoSP2 immunoblotted with P. ovis var. cuniculi-positive serum from rabbits with psoroptic mange, 8 purified rPso c 27 immunoblotted with anti-rPso c 27 immunoglobulin (IgG), 9 purified rPsoSP2 immunoblotted with anti-rPsoSP2 IgG, 10 purified rPso c 27 immunoblotted with P. ovis var. cuniculi-negative serum, 11 purified rPsoSP2 immunoblotted with P. ovis var. cuniculi-negative serum