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Table 2 Natural infection rates of Chrysops in the five study sites determined by microscopy and the loop-mediated isothermal amplification assay

From: Validation of loop-mediated isothermal amplification for the detection of Loa loa infection in Chrysops spp in experimental and natural field conditions

Study sites (years of MDA)

Microscopy method

Colorimetric LAMP assay

Fischer’s exact test (P-value)

Total number of wild-caught Chrysops flies screened

Number of positive flies (%)

Total number of wild-caught Chrysops of flies screened

Number of positive flies (%)

East MDA (9)

2365

61 (3.3)

183

30 (16.4)

0.000

East non-MDA (0)

1861

103 (4.4)

183

48 (26.2)

0.000

South-west 1 (15)

2318

66 (2.8)

434

137 (31.6)

0.000

South-west 2 (13)

812

10 (1.2)

200

1 (0.5)

0.105

North-west (10)

485

17 (3.5)

291

88 (30.2)

0.000

Total

7841

257 (3.3)

1291

304 (23.5)

0.000

  1. LAMP, Loop-mediated isothermal amplification; MDA , mass drug administration
  2. East MDA, Messamena Health District; East non-MDA, Batouri Health District north-west (NWA Health District); South-west 1, Kumba Health District; South-west 2, Mamfe Health District. See section Study sites for a detailed description