Fig. 6
Infected blood stored at 4 °C produces eL3. Infected blood was used fresh (0) or after storage for 1 or 2 days (1, 2) at 4 °C. a Graph of the average prevalence of D. immitis eL3 at 17 dpi in Ae. aegyptiS. The error bar indicates the SD. The number of mosquitoes (n) analyzed is indicated for each sample. Asterisks indicate a significant difference at P < 0.05 using Fisher’s exact test. b Blue dots are the number of D. immitis eL3 emerging from individual mosquitoes assayed at 17 dpi. Asterisks indicate significant differences in intensity using Kruskal–Wallis test with Dunn’s correction for multiple comparisons (*P < 0.05; **P < 0.01). Data are pooled from two independent experiments