Fig. 8

Emergence assay can be used to quantify B. malayi eL3. a Dots indicate the number of Brugia malayi microfilariae present in midguts of Ae. aegypti^S (blue) and Ae. aegypti^R (red) immediately following blood-feeding. Data in each column are not normally distributed; the black line is the median. The number of mosquitoes (n) analyzed is indicated for each sample. No significant difference was found using a Mann–Whitney test (P = 0.09). b Graph of the average prevalence of B. malayi eL3 assayed 12–14 dpi in Ae. aegypti^S (blue bars) and at 12 and 14 dpi in Ae. aegypti^R (red bars). Error bars indicate the SD. The number of mosquitoes (n) analyzed is indicated for each sample. No emerging parasites were observed for Ae. aegypti^R. There were no significant differences between the days assayed for Ae. aegypti^S, and all Ae. aegypti^S days assayed were significantly different from both Ae. aegypti^R on the two days assayed using an analysis of variance with Tukey’s multiple comparisons test, as indicated by asterisks (P < 0.001). c Blue dots are the number of B. malayi eL3 from individual mosquitoes assayed 12–14 dpi. No significant differences were found when all groups were compared to each other or when day 12 post infection was compared to the other groups using a Kruskal–Wallis test with Dunn’s correction for multiple comparisons. Data were pooled from three independent experiments