From: German Culex pipiens biotype molestus and Culex torrentium are vector-competent for Usutu virus
Blood meal virus titer (TCID50/ml) | Mosquito species | Dpi | Infection rate (%) (95% CI) | Mean viral load bodies (viral copies/µl of total RNA) | Dissemination rate (%) (95% CI) | Mean viral load legs plus wings (viral copies/µl of total RNA) | Transmission rate (%) (95% CI) |
---|---|---|---|---|---|---|---|
High titer 107.4 | Culex pipiens biotype molestusa | 14 | 8/10 (80.0) (44.4–97.5) | 6.9 × 105 | 3/8 (37.5) (8.5–75.5) | 9.0 × 103 | 3/3 (100) (29.2–100) |
21 | 4/6 (66.7) (22.3–95.7) | 5.6 × 105 | 4/4 (100) (39.7–100) | 1.5 × 104 | 3/4 (75.0) (19.4–99.4) | ||
Cx. pipiens biotype molestusb | 16 | 13/16 (81.3) (54.4–96.0) | 1.9 × 106 | 13/13 (100) (75.3–100) | 7.8 × 104 | 2/13 (15.4) (1.9–45.4) | |
21 | 8/10 (80.0) (44.4–97.5) | 8.1 × 105 | 8/8 (100) (63.1–100) | 7.8 × 104 | 4/8 (50.0) (15.7–84.3) | ||
Aedes aegyptid | 14 | 0/53 (0) (0–6.7) | NA | NA | NA | NA | |
21 | 4/22 (18.2) (5.2–40.3) | 2.3 × 105 | 1/4 (25.0) (0.6–80.6) | 5.5 × 103 | 0/1 (0) (0–97.5) | ||
Low titer 105.1 | Cx. pipiens biotype molestusa | 14 | 2/36 (5.6) (0.7–18.7) | 1.2 × 102 | 0/2 (0) (0–84.2) | NA | NA |
21 | 1/19 (5.3) (0.7–18.7) | 5.4 × 101 | 0/1 (0) (0–84.2) | NA | NA | ||
Cx. torrentiumc | 14 | 1/8 (12.5) (0.3–52.7) | 2.8 × 101 | 0/1 (0) (0–97.5) | NA | NA | |
21 | 1/8 (12.5) (0.3–52.7) | 3.9 × 106 | 1/1 (100) (2.5–100) | 4.7 × 104 | 1/1 (100) (2.5–100) |