Fig. 3

Expression of E. multilocularis sert and E. multilocularis tph in protoscoleces. a Whole mount in situ hybridization (WMISH) of E. multilocularis sert, b WMISH of E. multilocularis tph, c distribution of E. multilocularis sert-positive cells, d distribution of E. multilocularis tph-positive cells. Echinococcus multilocularis sert- and E. multilocularis tph-positive cells, respectively, were located in the rostellar ring (rr), the lateral ganglion (lg), the region of the anterior ring commissure (arc), the posterior lateral ganglion (plg), the lateral nerve cords (lnc), the medial nerve cords (mnc) and rarely in the posterior ring commissure (prc). a, b Micrographs are Z-projections (maximum intensity) of several focal planes. Bars: 25 µM. c, d Mean number of cells per protoscolex at each position (n = 18, isolates DPZ and MS1010). Error bars represent the standard deviation (SD)