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Table 1 Species and species-group identities assigned to Anopheles larvae based on mitochondrial and nuclear DNA sequences

From: Geographically extensive larval surveys reveal an unexpected scarcity of primary vector mosquitoes in a region of persistent malaria transmission in western Zambia

Final ID

Potential member species of group/complex (if applicable)

COI-assigned specimens (haplotypes)

Additional ITS2-assigned specimens

Dry season

Wet season

Total

An. coustani group

An. coustani s.s., An. cf. coustani 1, An. cf. coustani 2, An. tenebrosus, An. ziemanni

475 (203)

88

153

410

563

An. funestus

1 (1)

0

1

0

1

An. gambiae s.l.

An. arabiensis, An. gambiae s.s.

14 (6)

3

10

7

17

An. species O/15

42 (22)

0

1

41

42

An. squamosus

157 (79)

0

19

138

157

Unknown An. species

175 (71)

52

63

152

215

 

Total

864 (382)

143

247

748

995

  1. Identities inferred from above-threshold matches to cytochrome c oxidase I and/or internal transcribed spacer region 2 sequences on the National Center for Biotechnology Information nucleotide database (NCBI nt). Sampling undertaken in dry season (Sept–Oct 2017) and wet season (May–June 2018) in Limulunga and Mongu Districts, Western Province, Zambia. Of 3045 field-identified anophelines encountered, 1034 specimens were collected for genetic identification; 39 were subsequently identified as culicines and not reported in this table. “Unknown An. species” indicates below-threshold (<95%) matches to Anopheles sequences in NCBI nt; 12 specimens assigned to this group from COI sequences were subsequently re-assigned to other taxa based on ITS2 sequences