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Table 1 Species and species-group identities assigned to Anopheles larvae based on mitochondrial and nuclear DNA sequences

From: Geographically extensive larval surveys reveal an unexpected scarcity of primary vector mosquitoes in a region of persistent malaria transmission in western Zambia

Final ID Potential member species of group/complex (if applicable) COI-assigned specimens (haplotypes) Additional ITS2-assigned specimens Dry season Wet season Total
An. coustani group An. coustani s.s., An. cf. coustani 1, An. cf. coustani 2, An. tenebrosus, An. ziemanni 475 (203) 88 153 410 563
An. funestus 1 (1) 0 1 0 1
An. gambiae s.l. An. arabiensis, An. gambiae s.s. 14 (6) 3 10 7 17
An. species O/15 42 (22) 0 1 41 42
An. squamosus 157 (79) 0 19 138 157
Unknown An. species 175 (71) 52 63 152 215
  Total 864 (382) 143 247 748 995
  1. Identities inferred from above-threshold matches to cytochrome c oxidase I and/or internal transcribed spacer region 2 sequences on the National Center for Biotechnology Information nucleotide database (NCBI nt). Sampling undertaken in dry season (Sept–Oct 2017) and wet season (May–June 2018) in Limulunga and Mongu Districts, Western Province, Zambia. Of 3045 field-identified anophelines encountered, 1034 specimens were collected for genetic identification; 39 were subsequently identified as culicines and not reported in this table. “Unknown An. species” indicates below-threshold (<95%) matches to Anopheles sequences in NCBI nt; 12 specimens assigned to this group from COI sequences were subsequently re-assigned to other taxa based on ITS2 sequences