Fig. 2

Antibody response of the IgM (a) and IgG (b) subclass specific to Mesocestoides (M.) vogae homogenate (MvH) or excretory/secretory molecules (ES) in the peritoneal exudates of healthy and infected mice at 3, 6, 10, 14, 19, 25, 30 and 35 days post-infection (p.i.). The anti-M. vogae IgM and IgG antibody titers were determined by ELISA methods. Data are presented as the means of OD ± SD. OD values of IgM for uninfected mice (n = 3) were 0.059 ± 0.004 for MvH antigen and 0.067 ± 0.012 for ES. OD values of IgG for uninfected mice (n = 4) were 0.141 ± 0.025 for MvH antigen and 0.079 ± 0.013 for ES. The cut-off values are indicated in the figures. Statistical significance was analyzed using two-way ANOVA followed by Bonferroni's multiple comparison tests, and significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001. Detection of immunogenic antigens within MvH and ES preparations immunoreactive to IgG (c MvH; d ES) and IgM antibodies (e MvH; f ES) performed by western blot analysis. Exudates from infected mice obtained on corresponding days p.i. were incubated with nitrocellulose strips with blotted MvH or ES antigens.