Fig. 3
From: Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice
Peritoneal inflammation during Mesocestoides (M.) vogae infection is associated with an increased number of myeloid CD11b+Gr-1+ cells. Myeloid cell population in the peritoneal cavities of healthy and M. vogae-infected mice at 3, 6, 10, 14, 19, 25, 30 and 35 days post-infection. Peritoneal exudate cells (PEC) were isolated from the peritoneal cavity, and the expression of myeloid cell surface markers by these cells was analyzed using flow cytometry (FC). Live cells were gated on CD11b and Gr-1 expression to first identify CD11b+Gr-1+ cells. Then, F4/80 and MHC II expression was identified within the CD11b+Gr-1+ cells. a Representative dot plots obtained by FC analysis show the expression of CD11b/Gr-1 and F4/80/MHC II on PEC. b Proportions of CD11bhighGr-1+ cells in the peritoneal cavities of healthy and infected mice. c Proportions of F4/80+MHC II+ cells within CD11bhighGr-1+ cells in the peritoneal exudate cell population from healthy and infected mice. F4/80 (d) and MHC II (e) mean fluorescence intensity (MFI) within CD11bhighGr-1+ cells in the peritoneal exudate cell population from healthy and infected mice. Data are expressed as the mean ± SD. Statistical significance was analyzed using one-way ANOVA with Dunnett's post-test, and significantly different values between healthy and infected mice are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.