Skip to main content
Fig. 4 | Parasites & Vectors

Fig. 4

From: Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice

Fig. 4

Mesocestoides vogae infection regulates the production of inflammatory cytokines in LPS-induced peritoneal exudate cells (PEC). PEC were isolated from the peritoneal cavity of healthy and infected mice at 3, 6, 10, 14, 19, 25, 30 and 35 days post-infection. Then PEC were cultured in 24-well plates in the presence or absence of LPS (1 µg/ml) at 37 °C 5% CO2 for 72 h. The concentration of NO (a) in the culture supernatants was determined as nitrite (NO2) using Griess reagent. Nitrite concentration was determined using 0.1 M NaNO3 as the standard. Concentrations of cytokines TNF-α, IL-6 and IL-10 (b) in culture supernatants were measured by ELISA and expressed in pg/ml. Data are expressed as the mean ± SD. Statistical significance was analyzed using two-way ANOVA followed by Bonferroni's multiple comparison tests, and significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page