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Fig. 7 | Parasites & Vectors

Fig. 7

From: Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice

Fig. 7

Mesocestoides vogae excretory-secretory (ES) products induce the accumulation of CD11b+IL-10+ peritoneal cells. Mice were injected intraperitoneally with 20 µg of ES and PBS as control every day for a total of 6 days. Peritoneal exudate cells (PEC) were isolated and subjected to flow cytometric analysis. a Representative flow cytometry plots showing the expression of IL-10 and CD11b by PEC. Live cells were gated on CD11b expression to first identify CD11b+ cells. Then, IL-10 expression was identified within CD11b+ cells. b Proportions of CD11b+IL-10+ cells in the peritoneal cavities of PBS and ES treated mice. c Concentrations of IL-10 in the peritoneal exudates was measured by ELISA and expressed in pg/ml. d Relative expression of m-RNA for IL-10 in the peritoneal exudate cells was analyzed by real-time PCR. Data are expressed as the mean ± SD. Statistical significance was analyzed using Student's t test, and significantly different values between healthy and ES treated mice are indicated as: *p < 0.05, **p < 0.01.

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