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Fig. 2 | Parasites & Vectors

Fig. 2

From: The Eagle effect in the Wolbachia-worm symbiosis

Fig. 2

Time course experiment reveals antibiotics stop worm motility without a corresponding decrease in Wolbachia and vice versa. A time course experiment was conducted to determine the effects of doxycycline, tetracycline and rifampicin on B. pahangi females and Wolbachia titers at low (1 µM), intermediate (10 µM) and high (100 µM) concentrations at three time points (Day 1, 3 and 6). Doxycycline (a) and tetracycline (b) decreased worm motility but wsp/gst ratios did not fall in response to high antibiotic concentrations; at lower concentrations, worm motility was not impacted but wsp/gst ratios were reduced. Wolbachia titers were measured by wsp/gst ratios; medians with 95% confidence intervals are shown. X-axis labels show antibiotic concentration in µM. Percent differences in wsp/gst ratios compared to DMSO controls are shown below each antibiotic concentration. Negative percentages signify a decrease in Wolbachia titers, and positive percentages indicate that titers were higher than controls. Percent inhibition of motility is shown below each antibiotic concentration: 0% inhibition indicates that worms were as motile as controls, and 100% inhibition indicates that the worms were fully immotile. Red asterisks indicate statistical significance of the difference between wsp/gst ratios in treated worms and DMSO controls. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. Statistical significances of inhibition of motility are shown in Table S2 and a graphic illustration is shown in Fig. 3

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