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Fig. 5 | Parasites & Vectors

Fig. 5

From: The Eagle effect in the Wolbachia-worm symbiosis

Fig. 5

Wolbachia were depleted in the distal tip region of worm ovaries. Worms were treated with 10 µM doxycycline, minocycline, tetracycline and rifampicin for 6 days. Negative controls contained 1% DMSO in culture media. a Graph shows medians with 95% confidence intervals. ***P < 0.001; *P < 0.05. b Images of the distal tip region of B. pahangi ovaries from worms treated in vitro with tetracycline and rifampicin showing the elimination of Wolbachia in worm ovaries. Panels on the right are high magnification images of the boxed regions in the distal tip region. Wolbachia are the red puncta stained with propidium iodide and DAPI; the nuclei of host cells (worm cells) in the ovaries are stained blue/magenta by DAPI

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