| |
% Inhibition of motility
|
% Inhibition of formazan production
|
|---|
|
Compound
|
Day 1 (%)
|
Day 2 (%)
|
Day 3 (%)
|
Day 4 (%)
|
Day 6 (%)
|
Day 6 (%)
|
|---|
|
Doxycycline (100 µM)
|
54
|
94
|
97
|
99
|
99
|
91
|
|
Doxycycline (10 µM)
|
0
|
0
|
9
|
59
|
92
|
46
|
|
Doxycycline (1 µM)
|
0
|
0
|
0
|
2
|
0
|
8
|
- Worms treated with 100 and 10 µM doxycycline showed declining motility over time and were barely motile at 100 µM by Day 2. Viability was assessed on Day 6, as measured by formazan production in an MTT assay. The degree and duration of motility inhibition was predictive of reduced viability in the MTT assay
