Fig. 2
IL-33 deficiency is associated with transitory impairment of type 2 immunity in mesenteric lymph nodes (MLN) of infected mice. Female IL-33−/− and WT BALB/c mice (4–8 animals per group) were subcutaneously infected with 50 and 35 S. mansoni cercariae for 9 and 12 weeks, respectively, and sacrificed at 6, 9 and 12 wpi. Immune cells were isolated from MLNs and spleens of infected and non-infected (control) mice and stimulated with S. mansoni soluble egg antigens for 72 h at 37 °C, 5% CO2. IL-5 and IL-13 cytokines were measured in cell culture supernatants by enzyme-linked immunosorbent assay (ELISA). a IL-5 from MLNs. Comparison by unpaired two-tailed t-test with Welch’s correction for 6 wpi. b IL-13 from MLNs. c IL-5 from spleens. d IL-13 from spleens. Comparison by unpaired two-tailed t-test with Welch’s correction for 6 wpi. Data are representative of two independent experiments with similar results and are presented as the mean with SEM. Significance (P value) is indicated above connector bars between appropriate groups. Mouse groups were compared using the Mann-Whitney test at the P < 0.05 level of significance