Fig. 6

siRNAi gene knockdown of Dg vATPase A in D. gallinae. Quantitative PCR gene expression analysis of Dg vATPase A expression in adult female D. gallinae mites fed on Dg vATPase A siRNAs or lacZ siRNA (control). a Long R1 and R2 Dg vATPase A dsRNAs and long lacZ dsRNA were diced to produce short siRNAs and fed to adult female D. gallinae mites in goose blood (100 ng/μl). b siRNAs (27-mer) for Dg vATPase A and LacZ (control) were commercially synthesized and fed to adult female D. gallinae mites in goose blood (100 ng/μl). Dg vATPase A expression in both a and b is shown at 96 h post-feed and is normalised to GAPDH expression. Individual data points for biological replicates are shown with the mean ± SEM indicated (n = 3–6). No significant differences (ns) were detected between treatment groups using Student's t-test