Fig. 4.

Experimental confirmation and motif prediction of tritsRNAs. a Reverse transcription (RT)-PCR validation of tritsRNA candidates. Each tritsRNA is shown in four adjacent lanes, which are from left to right: stem-loop RT-PCR with DNase-treated RNA template (left, “+”); without RNA template (left middle, “-”); without reverse transcriptase in RT reactions (right middle, “-”); and without template in the PCR reaction as negative control (right, “-”). M indicates the DNA ladder. The yellow arrows refer to the products with the expected size. b Schematic representation of the motif sequences and locations in the backbone of a mature tRNA.