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Fig. 2 | Parasites & Vectors

Fig. 2

From: Clonorchis sinensis MF6p/HDM (CsMF6p/HDM) induces pro-inflammatory immune response in RAW 264.7 macrophage cells via NF-κB-dependent MAPK pathways

Fig. 2

Production of recombinant CsMF6p/HDM and anti-CsMF6/HDM. a Expression and purification of recombinant CsMF6p/HDM. Lane 1: E. coli lysate control; Lane 2: IPTG-induced E. coli lysate; Lane 3; CsMF6p/HDM purified by Ni-NTA affinity column. b Validation of anti-CsMF6p/HDM. The anti-CsMF6p/HDM was collected from mice immunized with CsMF6p/HDM and confirmed by immunoblot. Lane 1: normal serum; Lane 2: anti-CsMF6p/HDM

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