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Fig. 8 | Parasites & Vectors

Fig. 8

From: Clonorchis sinensis MF6p/HDM (CsMF6p/HDM) induces pro-inflammatory immune response in RAW 264.7 macrophage cells via NF-κB-dependent MAPK pathways

Fig. 8

Effect of NF-κB and AP-1 inhibitors on the expression of TNF-α and IL-6 in RAW 264.7 cells stimulated with CsMF6p/HDM. a RAW 264.7 cells were pretreated with different concentrations (0.1, 0.5, 1.0 μM) of NF-κB inhibitor (MG132) or AP-1 inhibitor (SR11302) for 3 h, and the cells were then incubated with CsMF6p/HDM (10 μg) and LPS (0.5 μg) for 12 h. The cells were harvested and the expression levels of TNF-α and IL-6 were analyzed by RT-PCR. Graphs show the densitometric ratios of TNF-α and IL-6 to GAPDH. b ELISA analysis. Cytokine production profiles were analyzed by quantitative ELISA

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