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Fig. 5 | Parasites & Vectors

Fig. 5

From: Leishmania braziliensis prostaglandin F synthase impacts host infection

Fig. 5

Detection of mChPGF2S in highly-infected macrophages. a Detection of mCherry fluorescence in the host cell cytoplasm. Time-lapse images of macrophages infected with more than three mChPGF2S parasites. Cyan was used as pseudocolor; numbers at the bottom right of each panel indicate hour:minutes after infection. b Close image analysis of mCherry and mChPGF2S localized fluorescence intensity in infected macrophages. Numbers at the top of each image indicate hour:minutes after infection. c Overall intracellular fluorescence in infected macrophages was quantified using FIJI (ImageJ) software up to 30 h pi. Scale-bars: a, 10 µm

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