Table 1 List of the primers designed for rnc operon characterization and Asaia sp. molecular identification
Primer name | Sequence (5ʹ–3ʹ) |
|---|---|
16S rRNA-F | TGGCGGACGGGTGAGTATC |
16s rRNA-R | AGTTGGTTTGACCCGAAGCC |
F566-3W | CTTTACCATCGCGGTTTCTGCTATG |
F624-3W | CGCCAGGCCGAGAGTGAG |
F652-3W | CCCTGCTTCGGCGATTGAAC |
R32-5W | TCGCAGAAGCCCCTGATCAG |
R161-5W | CAGACCGAGCACACGATCAC |
R182-5W | AAGCAGCCATTCAGCCATCAG |
F1 | GCCACCAGTTTGCTGATCAGG |
F2 | TGACGACTCATGACGCCATAGC |
F3 | CGTGTGCTCGGTCTGCTGATG |
F4 | CATATTGCGGAACATGAGGAGCAG |
F5 | GTTCAGGCCGACGCG |
R1 | ATGCCTTTACGGCCACGGTTCAATC |
R2 | GGCGGCGTGATCTGCGAG |
R3 | CCTCCATCGCGTCGGCCTG’ |
R4 | CAGCCTCACTCTCGGCCTG |
rnc-clF | AAGCTTGCATGACGACTCATGACGCCATAGCGGAG’ |
rnc-clR | CTCGAGTTTTTCTATCCTTCGTGCCTTTACGGCCAC |