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Fig. 3 | Parasites & Vectors

Fig. 3

From: Fowlerstefin, a cysteine protease inhibitor of Naegleria fowleri, induces inflammatory responses in BV-2 microglial cells in vitro

Fig. 3

Structure analysis of fowlerstefin. a Effect of SDS on fowlerpain structure. Fowlerstefin was incubated in different concentration of SDS (0–1 mM) at room temperature and separated by SDS-PAGE without heating. Lane M: siz parker proteins; Lane B: boiled sample in the presence of both SDS and β-ME. b Gel filtration chromatography. The Kav value of fowlerstefin (closed circles) was calculated and compared to those of standard marker proteins (open circles). The fractions with strong inhibitory activity against NfCPB-L (fractions 32 to 37, red bar) were further analyzed by SDS-PAGE. Lane M: size marker proteins

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