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Fig. 4 | Parasites & Vectors

Fig. 4

From: Fowlerstefin, a cysteine protease inhibitor of Naegleria fowleri, induces inflammatory responses in BV-2 microglial cells in vitro

Fig. 4

Expression profile of fowlerstefin. a Morphological changes. Morphological changes of N. fowleri trophozoites into precysts (or cysts) cultured in encystation media for 0, 6, 12, 24, 36, 48 and 72 h. b RT-PCR analysis. The expression profiles of fowlerstefin, NfCPB, NfCPB-L and NfGAPDH were analyzed in different developmental stages of N. fowleri. Graphs show the densitometric ratios of fowlerstefin, NfCPB and NfCPB-L to NfGAPDH. c Immunoblot analysis. Expression pattern of fowlerstefin in cysts and trophozoites was analyzed by immunoblot with anti-fowlerstefin (upper blot). The ESP and cell lysates of N. fowleri trophozoies were analyzed by immnoblot with anti-fowlerstefin (lower blot)

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Parasites & Vectors

ISSN: 1756-3305

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