Fig. 5

Immunofluorescence analysis of Hc-HRG-2 in adult H. contortus. Neutral Hc-HRG-2 was detected using mouse anti-Hc-HRG-2 polyclonal antibody and fluorophore-conjugated anti-mouse IgG antibodies in the paraffin section of worms. a Mouse anti-Hc-HRG-2 polyclonal antibody recognized the natural protein of adult H. contortus (black asterisks, target band). b A negative control using pre-immune serum as primary antibody. Lane 1: PBS-soluble natural protein; Lane 2: urea-soluble natural protein. c Cross-section of a female adult (scale-bars: 50 μm). f Cross-section of a male adult (scale-bars: 20 μm). Merge, GFP, DIC and DAPI labeled as 1, 2, 3 and 4, respectively. d, e and g represent the magnified parts of the sections (scale-bars: 10 μm). Key: triangles, intestine; asterisks and circles, gonad; white arrows, body hypodermal tissues