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Fig. 6 | Parasites & Vectors

Fig. 6

From: Hc-hrg-2, a glutathione transferase gene, regulates heme homeostasis in the blood-feeding parasitic nematode Haemonchus contortus

Fig. 6

The rHc-HRG-2-heme complex exhibits peroxidase activity. Purified proteins were incubated with heme at 37 °C for 2 h. Thereafter, the formed complexes were resolved by native PAGE. a The presence of heme revealed by TMB (triangle: target band). b The presence of protein by Coomassie blue staining (asterisks: target bands for rHc-HRG-2; dot: target band for equine heart myoglobin). Lane 1: rHc-HRG-2 only; Lane 2: rHc-HRG-2 plus heme; Lane 3: the positive control, equine heart myoglobin

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