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Fig. 7 | Parasites & Vectors

Fig. 7

From: Hc-hrg-2, a glutathione transferase gene, regulates heme homeostasis in the blood-feeding parasitic nematode Haemonchus contortus

Fig. 7

Heme-binding and GST enzymatic activities of rHc-HRG2. a UV-visible absorption spectra recorded in the presence of 5 μM rHc-HRG2 and 6 μM heme. b Difference absorption spectra recorded from the heme titration experiment using 5 μM rHc-HRG2 and various concentrations of heme ranging from 2 to 14 μM. c Heme-binding curve generated from the difference absorption spectra in panel b by plotting ∆A414 versus the heme concentration. The results represent the mean ± SE of three independent experiments. d The GST activity of rHc-HRG-2 (10 μM), representing the mean ± SE of triplicates. e Inhibition of GST activity by the binding of heme (10 μM) to rHc-HRG-2 (10 μM). *P < 0.05

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