Table 2 Sequences of different primer sets used for RNAi
Gene | Primer sequence (5ʹ-3ʹ) | Amplicon size (bp) |
|---|---|---|
Cathepsin B (T7) | F: TAATACGACTCACTATAGGATTGTCCACCTCGCTGCC | 430 |
R: TAATACGACTCACTATAGGGGTCCGTGTGCCTCTGGT | ||
Cathepsin D (T7) | F: TAATACGACTCACTATAGGTGTTCGACACCGGCTCCT | 497 |
R: TAATACGACTCACTATAGGCTGCCAGTAGCCCTTGCG | ||
Acid phosphatase (T7) | F: TAATACGACTCACTATAGGGGTCACATCACGCACAAA | 539 |
R: TAATACGACTCACTATAGGTGCAGGGTGCTGTTGTAG | ||
GFP (T7) | F: TAATACGACTCACTATAGGGACGTAAACGGCCACAAGT | 583 |
R: TAATACGACTCACTATAGGGCTTCTCGTTGGGGTCTTT |