Fig. 1

Transfection and drug screening of the PfSWIB fusion expression system. a Schematic illustration of conditional PfSWIB knockdown strategy in the 3D7 clone. The pLN-HA-FKBP-LID construct was derived from plasmid pLN-ENR-GFP [44], which harbored an antibiotic selection marker (bsd for Blasticidin S resistance, pink). HA tags (red) and FKBP-LID tag (blue and green) were ligated towards the 1.5 kb C terminus of PfSWIB. b Drug screening of the PfSWIB fusion expression system. The integration events and expression of PfSWIB modified locus were identified by PCR. ‘BSD+’ and ‘BSD-’ indicate that the transfected parasites were cultured in the presence or absence of BSD. Day 24: in vitro culturing in the absence of BSD for 24 days. Day 5, 10 and 15: in vitro culturing in the presence of BSD for 5, 10 and 15 days, respectively. The details of specific primers used for detecting the endogenous, episomal and integrated PfSWIB locus are provided in “Methods”