Fig. 3

Dynamic studies of CD3ζ expression in CD4⁺ and CD8⁺ T cells in vivo. a Representative flow cytometry analysis of CD3ζ expression in CD4⁺ (CD3ε⁺CD4⁺) and CD8⁺ (CD3ε⁺CD8⁺) T cells among spleen leukocytes from one infected mouse at 6 months post-infection. b The relative MFI of CD3ζ expression in CD4⁺ and CD8⁺ T cells was quantified by flow cytometry at different time points post-infection (3, 6, 9 and 12 months). The differences were analyzed by a two-way ANOVA. The data are the result of one representative experiment out of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: ns, not significant