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Fig. 4 | Parasites & Vectors

Fig. 4

From: Comparison of immune responses to Loa loa stage-specific antigen extracts in Loa loa-exposed BALB/c mice upon clearance of infection

Fig. 4

Adult worm and MF antigen extract induce distinct chemokines. Groups of wildtype BALB/c mice were either subcutaneously infected with larvae (L3, L4), i.v. injected with MF or were implanted with L5 or adult worms. On day 42 pi, lymphocytes isolated from infected BALB/c mice were left unstimulated or cultured with 100 µg/ml of the Loa loa developmental stage antigen extract that was originally used to infect the mouse. Antigen stimulation in cell cultures of lymphocytes from naive BALB/c mice served as control. Re-stimulation assays were cultured for 48 h at 37 °C and 5% CO2 and resulting levels of GM-CSF (a), CXCL10 (b) and CXCL1 (c) were determined using the Luminex technology. Data show concentration (pg/ml) of the different chemokines from re-stimulated splenocytes of infected BALB/c mice (closed symbols, n = 6 per life stage/parasite antigen extract) and naive BALB/c mice (open symbols, n = 3 per parasite antigen extract). Chemokine levels were normalized by subtracting background levels of the comparable un-stimulated controls. Graphs show scatter plots with mean ± SEM. Statistically significant differences between the indicated groups were detected using the Friedman test followed by a Dunn’s multiple comparison test

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