Fig. 3
Anti-Trichomonas vaginalis assay. MIC and IC50 for the antiparasitic activity of PFUR 4a (a) and PFUR 4b (b) against Trichomonas vaginalis ATCC 30236 after exposure to 1.5625, 3.125, 6.25, 12.5, 25, 50 and 100 µM concentrations for 24 h. Kinetic growth curves of Trichomonas vaginalis ATCC 30236 after 1, 6, 12, 24, 48, 72 and 96 h of treatment with PFUR 4a (c) and PFUR 4b (d) at 6.25 µM. Growth was completely inhibited after 24 h. Control (untreated trophozoites), 0.6% DMSO (vehicle for solubilization), MTZ (metronidazole at 100 µM). Viability of 100% in control corresponds to 2.6 × 105 trophozoites/ml. Data are presented as the mean ± standard deviation of at least three independent experiments. Different letters show a significant difference at P < 0.05