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Table 2 Details of species-specific assays for S. mansoni and S. haematobium

From: Environmental DNA-based xenomonitoring for determining Schistosoma presence in tropical freshwaters

Species

Primer or probe

Sequence (5′-3′)

Product length (bp)

S. mansoni

Forward primer

CTGCTCAGTGAAGAAGTTTGTTT

104

Probe

AGCCGCGATTATTTATCGTGCTAAGGT

Reverse primer

CCTCATTGAACCATTCACAAGTC

S. haematobium

Forward primer

AATGAACATGAATGGCCGCA

143

Probe

TGGAGACTTGTGAATGGTCGAACG

Reverse primer

ATGGGTTCCTCACCACTTAAACT

  1. Notes: Probes were designed with a dual labelled 5(6)-carboxy-fluorescein (FAM) fluorescent tag at the 5′ end and with a black hole quencher 1 (BHQ1) at the 3′-end