Fig. 5
Estimation of cytokines, NO and H2O2 in culture supernatants of RAW 264.7 by ELISA. Levels of interleukin 12 (IL-12) (a), interferon γ (IFN-γ) (b), tumor necrosis factor α (TNF-α) (c), interleukin 10 (IL-10) (d), interleukin 4 (IL-4) (e), nitric oxide (NO) (f) and hydrogen peroxide (H2O2) (g) production in culture supernatants was measured. Abbreviations: N, non-infected macrophages; I-L. DD8, macrophages infected with L. DD8; I-L. DD8 + SSG, SSG adding after macrophages infected with L. DD8; I-L. DD8 + CsA, CsA adding after macrophages infected with L. DD8; I-L. DD8 + DHCsA-d, DHCsA-d adding after macrophages infected with L. DD8. Data are the mean ± standard error of the mean and represent the values of one of the 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001