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Fig. 6 | Parasites & Vectors

Fig. 6

From: Evaluation of in vitro antileishmanial efficacy of cyclosporin A and its non-immunosuppressive derivative, dihydrocyclosporin A

Fig. 6

Cloning and expression of LdCyPA protein. a SDS-PAGE analysis of fusion protein in Escherichia coli BL21 (containing pET28a). Lane M: protein marker; Lane 1: total protein before induction; Lane 2: 20 °C supernatant; Lane 3: 20 °C precipitate; Lane 4: 37 °C supernatant; Lane 5: 37 °C precipitate (target fusion protein). b SDS-PAGE analysis of eluted protein. Lane M: protein marker; Lane 1: sample solution; Lane 2: penetrating fluid; Lane 3: 20 mM Imidazole-eluted protein; Lane 4: 50 mM Imidazole-eluted protein; Lane 5: 500 mM Imidazole-eluted protein (target LdCyPA protein). c Western blotting-based identification of the purified LdCyPA protein. Lane M: protein markers; Lane 1: purified LdCyPA protein

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