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Fig. 7 | Parasites & Vectors

Fig. 7

From: Evaluation of in vitro antileishmanial efficacy of cyclosporin A and its non-immunosuppressive derivative, dihydrocyclosporin A

Fig. 7

LdCyPA and CyPA expression after treatment with three drugs. a LdCyPA expression levels of promastigotes treated with SSG, DHCsA-d, or CsA for 48 h; LdCyPA polyclonal antibody and α-tubulin antibody were used as primary antibodies. b LdCyPA expression levels of intracellular amastigotes treated with SSG, DHCsA-d, or CsA for 48 h; LdCyPA polyclonal antibody and α-tubulin antibody used as primary antibodies. c CyPA expression levels of RAW 264.7 mouse macrophages co-cultured with SSG, DHCsA-d, or CsA at 48 h; CyPA and α-tubulin antibodies used as primary antibodies. Densitometry plot of LdCyPA expression in promastigotes (d) and amastigotes (e) normalized by an endogenous control, α-tubulin. f Densitometry plot of CyPA expression in RAW264.7 cells, normalized by an endogenous control, α-tubulin. Data are represented as the mean ± SD and represent the values of one of the 3 independent experiments. ***P < 0.001, ****P < 0.0001. Abbreviations: A, intracellular amastigote; P, promastigote

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