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Fig. 2 | Parasites & Vectors

Fig. 2

From: Vascular wall injury and inflammation are key pathogenic mechanisms responsible for early testicular degeneration during acute besnoitiosis in bulls

Fig. 2

Besnoitia besnoiti tissue cysts in scrotal skin, testicular parenchyma and pampiniform plexus. ac Histopathology: H&E staining in which cyst is occluding a vessel in testicular parenchyma from bull 2 (a ×400); histological section of scrotal skin stained with PAS staining in which tissue cysts of B. besnoiti are observed in the papillary layer of the dermis (b, c ×100). The black arrows indicate the bradyzoites and the yellow arrow the nuclei of the host cell. df Immunohistochemistry: staining of tissue section from bull 6 with rabbit-α-B. besnoiti showed the presence of particulate antigen of the parasite associated with vasculitis (amorphous granular debris positively labelled) in scrotal skin (d ×200). All tissue samples from bull 2 presented cysts with irregular distribution (e, f), the number of cysts being greater in the scrotal skin (e) than in the testicular parenchyma and pampiniform plexus (f). Scale-bars: a, b, f 20 µm; c, e 100 µm; d 50 µm

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