Fig. 2From: Vascular wall injury and inflammation are key pathogenic mechanisms responsible for early testicular degeneration during acute besnoitiosis in bullsBesnoitia besnoiti tissue cysts in scrotal skin, testicular parenchyma and pampiniform plexus. a–c Histopathology: H&E staining in which cyst is occluding a vessel in testicular parenchyma from bull 2 (a ×400); histological section of scrotal skin stained with PAS staining in which tissue cysts of B. besnoiti are observed in the papillary layer of the dermis (b, c ×100). The black arrows indicate the bradyzoites and the yellow arrow the nuclei of the host cell. d–f Immunohistochemistry: staining of tissue section from bull 6 with rabbit-α-B. besnoiti showed the presence of particulate antigen of the parasite associated with vasculitis (amorphous granular debris positively labelled) in scrotal skin (d ×200). All tissue samples from bull 2 presented cysts with irregular distribution (e, f), the number of cysts being greater in the scrotal skin (e) than in the testicular parenchyma and pampiniform plexus (f). Scale-bars: a, b, f 20 µm; c, e 100 µm; d 50 µmBack to article page