Genetic differentiation and population structure of Anopheles funestus from Uganda and the southern African countries of Malawi, Mozambique, Zambia and Zimbabwe

Kaddumukasa, Martha A.; Wright, Jane; Muleba, Mbanga; Stevenson, Jenny C.; Norris, Douglas E.; Coetzee, Maureen

doi:10.1186/s13071-020-3962-1

Table 2 Primer sequences and characteristics of 12 polymorphic microsatellite loci

From: Genetic differentiation and population structure of Anopheles funestus from Uganda and the southern African countries of Malawi, Mozambique, Zambia and Zimbabwe

Locus	Chromosome arm	Repeat motif	No. of alleles	Allele size	Primer sequence (5′-3′)	Reference
FunL F	2L	(GT)₈	12	181–197	HEX-AACAGTGGAAGGCAAATTGC	Cohuet et al. [22]
FunL R	2L	(GT)₈	12	181–197	GCACGGTTACCACTGCTCA	Cohuet et al. [22]
AFUB10 F	2L	(GCT)_3+2+4+5	6	195–210	PET-TGTCCATGTACAACCGCAAC	Sharakov et al. [23]
AFUB10 R	2L	(GCT)_3+2+4+5	6	195–210	TTCTCCAGCATCATCAGCAC	Sharakov et al. [23]
AFUB11 F	2L	(CTG)_3+5+2+2	4	188–191	PET-CAGTTTCTGCGTGGAGGAAT	Sharakov et al. [23]
AFUB11 R	2L	(CTG)_3+5+2+2	4	188–191	AGCAGCTGATGAGCCATCTC	Sharakov et al. [23]
AFND23 F	2L	(GT)₁₁	11	133–157	6-FAM-TTTGATCGACGGACTAGTGTGT	Schemerhorn et al. [25]
AFND23 R	2L	(GT)₁₁	11	133–157	GGTTTGATGGGTGGAAAC	Schemerhorn et al. [25]
FunO F	2R	(CA)₆T(AC)₄	10	110–132	PETGCACACATTTCAGGCAGC	Cohuet et al. [22]
FunO R	2R	(CA)₆T(AC)₄	10	110–132	GCCCACATTCTGCACCTT	Cohuet et al. [22]
AFUB3 F	2R	(CAG)₂₊₃₊₂	5	171–195	VIC- GGGAAGGATTCGACCTTAGC	Sharakov et al. [23]
AFUB3 R	2R	(CAG)₂₊₃₊₂	5	171–195	GCCGCCATTTAGTAGCAGTT	Sharakov et al. [23]
FunF F	3L	(TG)₉	7	104–118	6-FAM –GCCTTCAGTTTCGATTGGCG	Cohuet et al. [22]
FunF R	3L	(TG)₉	7	104–118	AATAAGATGCGACCGTGGC	Cohuet et al. [22]
AFUB12 F	3L	(AGG)₇(TG)₄	3	152–158	VIC –TGGGGAACTGGTCGTTAGAG	Sharakov et al. [23]
AFUB12 R	3L	(AGG)₇(TG)₄	3	152–158	CTGGTGATGGGATTGAGGAT	Sharakov et al. [23]
AFND19 F	3R	(AG)₁₂(TG)₅	8	251–285	HEX –GCAAGCTGTACGCAGAGAG	Sharakov et al. [24]
AFND19 R	3R	(AG)₁₂(TG)₅	8	251–285	ATCGATGGGAGTTATTATACGC	Sharakov et al. [24]
AFND20 F	3R	(GAG)_4+2+2+2(TGG)₃	2	239–242	VIC- CGGCGCAGGTTTAGTAGC	Sharakov et al. [24]
AFND20 R	3R	(GAG)_4+2+2+2(TGG)₃	2	239–242	CCCTCGCTTTCCTCATAAAA	Sharakov et al. [24]
AFND41 F	3R	(CA)₃TC(CA)₆	6	222–246	6-FAM AGAACATATGGCAAATCGAC	Schemerhorn et al. [25]
AFND41 R	3R	(CA)₃TC(CA)₆	6	222–246	GAAAGACTTGTCGGACGTG	Schemerhorn et al. [25]
FunQ F	X	(TG)₉	7	84–98	HEX –GCAAACTGCTAGTAAATGTTTCC	Cohuet et al. [22]
FunQ R	X	(TG)₉	7	84–98	ACATTTCCACAATTTGCGC	Cohuet et al. [22]

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ISSN: 1756-3305

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