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Table 1 List of proteins identified by GeLC-MS/MS analysis of A. foliaceus secretory/excretory products showing hits with OmicsBOx (Blast2Go) database

From: Identification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus, 1758) reveals unique secreted proteins amongst haematophagous ecdysozoa

Family/Domain SeqName Description MW (kDa) No. Peptides SC (%) Pro. length E-value SignalP Hits Organism Function NCBI ID
Vitellogenin_N afol_3429.1p Von Willebrand factor type D domain protein 172.5 55 39.4 1499 8.39E−11 Yes Ixodes scapularis Transporters, osmolality, immunity and clotting [25, 51,52,53] XP_029822532.1
afol_51180.1p Vitellogenin-like isoform X1 183.8 44 30.1 1582 1.26E−11 No Ixodes scapularis XP_029826448.1
Hemocyanin afol_43548.1p Hemocyanin subunit type 1 precursor 81.2 18 26.3 697 0 Yes Argulus foliaceus Respiratory, protein storage [54] CUH82791.1
afol_52001.1p Hemocyanin A chain 36.2 8 30.6 314 0 No Argulus foliaceus CUH82792.1
Astacin afol_16671.1p Protein SpAN-like 72.6 11 21.2 660 1.49E−55 Yes Saccoglossus kowalevskii Immunity; antifungal activity [55], food digestion, host penetration, immune evasion or activation [56,57,58] XP_002739691.1
afol_52344.2p Protein SpAN-like 76.4 12 17.6 687 2.85E−50 No Penaeus vannamei XP_027221531.1
afol_18725.8p Blastula protease 10-like 50.7 8 18.4 462 1.44E−17 No Penaeus vannamei XP_027219989.1
Serpin afol_27409.2p Serpin B6-like 51.4 7 20.0 461 6.15E−66 No Rhipicephalus microplus Anticoagulation activity, modulate host immune response, regulation of host inflammation, antihaemostatic effects, and platelet aggregation [59,60,61,62,63,64] AHC98669.1
afol_25414.2p Leukocyte elastase inhibitor-like 11.2 4 35.1 94 1.05E−16 No Ixodes scapularis EEC05896.1
Fasciclin afol_20127.1p Beta-ig-h3 fasciclin 37.1 5 23.1 333 1.64E−22 Yes Culex quinquefasciatus Mediate cell adhesion [65, 66] XP_001847648.1
Trypsin afol_3246.7p Transmembrane protease serine 9 28.1 5 21.4 262 2.81E−12 No Penaeus vannamei Digestion, maintain host-parasite relationship [67] XP_027224040.1
afol_16878.20p Trypsin-1 32.0 9 34.5 293 6.81E−61 Yes Ceratitis capitata XP_004520346.1
afol_19181.1p Serine protease 29 47.0 7 24.5 466 8.72E−87 Yes Bombus terrestris XP_020722765.1
afol_18345.1p Transmembrane protease serine 9-like 18.3 4 25.6 164 1.38E−17 No Drosophila navojoa XP_030246706.1
afol_51127.2p Trypsin 25.1 3 15.7 236 2.13E−57 No Penaeus vannamei ROT79324.1
afol_56688.3p Serine protease1/2 29.1 2 6.1 262 3.42E−39 Yes Ixodes scapularis XP_029851465.1
Peptidase_M14 afol_12392.3p Mast cell carboxypeptidase A 48.3 5 16.7 424 4.21E−88 No Penaeus vannamei Proteolytic-enzyme [68]) XP_027226524.1
VIT afol_32337.1p Inter-alpha-trypsin inhibitor heavy chain H4-like isoform X2 98.2 4 7.4 883 2.31E−151 Yes Lingula anatina Proteinase inhibitor [36] XP_013407760.1
  1. Note: Description of suggested functions reported in different species of arthropods, and were blasted against Metazoa in NCBI BLASTP to obtain an Accession No. Signal P was used to predict the secretory property of the protein
  2. Abbreviations: MW, molecular weight of protein; SC, sequence coverage; E-value, from the OmicsBox output