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Table 1 Immunoproteomic and mass-spectrometric identification of Eimeria acervulina sporozoite proteins recognized by immune sera of the three Eimeria species studied

From: Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Spot IDaIdentified protein grouped by functionsAccession numberbNo. of matched peptidescTheoretical Mr/pIProtein scoredProtein sequence coverage (%)e
Protein translation
 1Elongation factor 1-beta, putative, partialXP_013248731.116/4019289/4.5120962
 6Eukaryotic translation initiation factor 3 subunit 8, putativeXP_013250573.114/3731924/9.7617255
Transcription
 8Elongin c, putativeXP_013249709.111/4717120/5.3414449
Cargo transport and protein trafficking
 2Intraflagellar transport particle protein, putative, partialXP_013246990.116/4628884/8.3522043
 9AP-4 complex subunit sigma-1, putativeXP_013248843.111/3317189/5.4113540
Ribosome-related proteins
 760S ribosomal protein L8, putativeXP_013249139.112/4028042/10.7112037
 18Ribosome biogenesis protein brix, putativeXP_013248150.117/5454150/9.8217038
Predicted domain-containing proteins
 3Uncharacterized ACR, YagE family COG1723 domain-containing protein, putativeXP_013250980.118/4520048/9.5824049
 4U1 like C2H2 zinc finger, relatedXP_013248237.114/3528894/9.6019968
Chaperone proteins
 17DnaJ domain-containing protein, putativeXP_013253345.115/4948311/6.5614036
Protein of enzymatic properties
 5Nucleolar GTP-binding protein, putativeXP_013252320.111/4121119/9.7014757
 10Glycerol-3-phosphate dehydrogenase, putative, partialXP_013248247.117/3135465/6.6723760
 11Protein phosphatase 2C, putativeXP_013248554.113/5642702/5.8211628
 12Glyceraldehyde-3-phosphate dehydrogenase, putativeXP_013251305.116/4636753/7.5721141
 13Cysteine desulfurase, putativeXP_013251976.117/3463362/8.4319145
 14Glyoxalase, putativeXP_013247421.120/3965222/6.6324637
 15Phosphatase, putativeXP_013251081.114/3862536/9.3615834
 16Phosphoribosylpyrophosphate synthetase, putativeXP_013248652.117/4047500/9.3017157
 19CAM kinase, CDPK family TgPK1, putativeXP_013250395.115/36118100/7.1213416
 20DNA photolyase, putativeXP_013251340.114/3152839/8.7914737
 21gamma-glutamyl phosphate reductase, putativeXP_013252670.112/3444087/5.4615336
  1. aProtein spot numbering in the 2-DE gel; spots 22 and 23 that had no detectable protein signals are not listed
  2. bAccession number in NCBI
  3. cNumber of peptides that match the predicted protein sequence
  4. dProtein score is − 10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 71 are significant (P < 0.05)
  5. ePercentage of predicted protein sequence covered by matched peptides