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Table 2 Eimeria protein amino acid sequence comparison

From: Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Spot IDaAccession numberAmino acid sequence identity (%)bSecP/SP/TM predictionc
E. acervulinaE. tenellaE. necatrixd
1XP_013248731.110058.7244.880.053/N/N
2XP_013246990.110051.6552.070.656*/N/N
3XP_013250980.110038.9541.180.068/N/N
4XP_013248237.110048.5448.710.371/N/N
5XP_013252320.110075.000.533/N/N
6XP_013250573.110075.1975.190.843*/N/N
7XP_013249139.110095.1494.330.462/N/N
8XP_013249709.110061.0762.090.798*/N/N
9XP_013248843.110089.5885.420.435/N/N
10XP_013248247.110073.6773.350.42/N/N
11XP_013248554.110050.0051.800.662*/N/N
12XP_013251305.110094.1092.630.635*/N/N
13XP_013251976.110066.5466.730.404/N/N
14XP_013247421.110058.4460.600.245/N/N
15XP_013251081.110080.9581.280.172/N/N
16XP_013248652.110084.5689.520.799*/N/N
17XP_013253345.110096.0096.000.283/N/N
18XP_013248150.110061.9062.670.423/N/N
19XP_013250395.110061.6847.850.298/N/N
20XP_013251340.110058.0259.360.376/N/N
21XP_013252670.110067.2667.510.454/N/N
  1. a2-dimensional gel electrophoresis (2-DE) gel spot number
  2. bAll protein sequences within the same row are compared to that of E. acervulina using CLUSTAL Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/)
  3. cNon-classical secretory property (SecP) and presence of a signal peptide (SP) and transmembrane domains (TM) were analyzed by the SecretomeP 2.0a Server (http://www.cbs.dtu.dk/services/SecretomeP/), SignalP-5.0 Server (http://www.cbs.dtu.dk/services/SignalP/) and TMHMM Server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM/), respectively
  4. dEimeria necatrix ortholog for XP_013252320.1 was not found
  5. Abbreviations: *, a SecP score exceeds the threshold (0.6), indicative of a secretory potential through the non-classical pathway for proteins with no signal peptides; N, not present