Borrelia miyamotoi and Borrelia burgdorferi (sensu lato) identification and survey of tick-borne encephalitis virus in ticks from north-eastern Germany

Răileanu, Cristian; Tauchmann, Oliver; Vasić, Ana; Wöhnke, Elisabeth; Silaghi, Cornelia

doi:10.1186/s13071-020-3969-7

Table 2 Primer pairs used for detection or for the MLST of selected targets of Borrelia spp. in I. ricinus ticks

From: Borrelia miyamotoi and Borrelia burgdorferi (sensu lato) identification and survey of tick-borne encephalitis virus in ticks from north-eastern Germany

Pathogen	Target	Primer/Probe	Sequence (5′-3′)	References
Borrelia spp.	16S-23S rRNA	Bospp-IGS-F	GTATGTTTAGTGAGGGGGGTG	[32]
		Bospp-IGS-R	GGATCATAGCTCAGGTGGTTAG
		Bospp-IGS-Fi	AGGGGGGTGAAGTCGTAACAAG
		Bospp-IGS-Ri	GTCTGATAAACCTGAGGTCGGA
B. miyamotoi	nifS	BmnifF31	GAAAAAGTAAACTCCCTCAGAAAGG	[35]
	nifS	BmnifR892	CAATGATGCCTGCAATATTTGGTG	[35]
	clpA	BmclpAF1268	TTGATCTCTTAGATGATCTTGG	[35]
	clpA	BmclpAR2051	CAAACATAAACCTTTTCAGCCTTTAATA	[35]
	rlpB	BmrplF18	ATTAAGACTTATARGCCAAAAAC	[35]
	rlpB	BmrplR761	GGCTGNCCCCAAGGWGAT	[35]
	pyrG	BmpyrF415	CTTYTAGTWATTGARATTGGTGGT	[35]
	pyrG	BmpyrR1261	CAGCATCAAYTATRCCACAAAC	[35]
	recG	BmrecF908	CTAGYATTCCTYTAATTGAGGC	[35]
	recG	BmrecR1779	TTCRGTTAAAGGTTCCTTATAAAG	[35]
	clpX	BmclpXF104	CTGTTGCYATTTGTTTTGAATGC(Y)TC	[35]
	clpX	BmclpXR1277	TAAAGTTCTTTTGCCCAAGG	[35]
	pepX	BmpepXF361	AGAGAYTTAAGYTTAKCAGG	[35]
	pepX	BmpepXR1202	GTTTCTCTTAAAGAYTGCATTCC	[35]
	uvrA	BmuvrF1435	GCTKAAATTTTTRATTGATGTTGGA	[35]
	uvrA	BmuvrR2306	CARGGAACAAAAACATCRGGC	[35]
	rplB^a	BmrplF23	GACTTATAGGCCAAAAACTTC	[35]
	rplB^a	Bmrpl759R	GATACAGGATGACGACCACC	[35]
	pyrG^a	BmpyrF417	TTTAGTAATTGAGATTGGTGGTAC	[35]
	pyrG^a	Bmpyr1252R	TATTCCACAAACATTACGAGC	[35]
	recG^a	BmrecF909	TAGCATTCCTTTAGTTGAGGC	[35]
	recG^a	Bmrec1671R	CTCAGCATGCTCAACTACC	[35]
	clpX^a	BmclpF268	TTATCTGTTGCTGTTTATAATC	[35]
	clpX^a	BmclpX1155R	TTCAAACATAACATCTTTAAGTAATTCTTC	[35]
	pepX^a	BmpepF361	AGAGACTTAAATTTAGCAGGAGTTG	[35]
	pepX^a	Bmpep1187R	TGCATTCCCCACATTGGAGTTC	[35]
	uvrA^a	BmuvrF1437	TTAAATTTTTAATTGATGTTGGACT	[35]
	uvrA^a	Bmuvr2147R	TCTGTAAAAAACCCAACATAAGTTGC	[35]
TBEV	3′ non-coding region	F_TBE_1	GGGCGGTTCTTGTTCTCC	[36]
		R_TBE_1	ACACATCACCTCCTTGTCAGACT
		TBE-probe-WT	TGAGCCACCATCACCCAGACACA

^aSequencing primers used in the second round of amplification and for sequencing the PCR products

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ISSN: 1756-3305

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