Fig. 1

Comparison of the normalized relative quantity of LdMT (chromosome 13) and LdROS3 (chromosome 32) for BPK275/0cl18 (a) and BPK282/0cl4 (b). A significant reduction in LdMT relative quantity could be detected between the WT and the in vitro and in vivo MIL-selected lines, and is compatible with WT being trisomic and the MIL-selected line disomic for chromosome 13 (arrows). A significant difference in LdMT could also be noted for the WT strain before and after passage in the sand fly. No significant differences could be detected in LdROS3 gene copy number for the in vivo-selected. Results are based on duplicate measurements of at least three biological replicates and are expressed as the mean (± SE) relative quantity normalized using chromosome 24 (stably disomic), chromosome 31 (mostly tetrasomic) and chromosome 36 (stably disomic) as references. **P < 0.01, ***P < 0.001, ****P < 0.0001