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Fig. 2 | Parasites & Vectors

Fig. 2

From: Phenotypic adaptations of Leishmania donovani to recurrent miltefosine exposure and impact on sand fly infection

Fig. 2

(a) Flow cytometric analysis of BODIPY-MIL-labelled promastigotes of BPK275/0-cl18 WT. A-1: Side scatter (SSC-H) versus forward scatter (FSC-H) dot plot with gated population corresponding to 0.5 µM MIL-treated promastigotes. A-2: Further characterization of promastigotes based on live/dead staining TO-PRO-3 and BODIPY-MIL fluorescence. A-3: Non-treated promastigotes (control sample). Excitation was set at 488 nm and emission was detected by FL2 channel. Flow cytometric analysis of BODIPY-MIL accumulation within 1 h exposure in log (96 h) and stationary-phase (168 h) promastigotes of the WT parent and in vitro (b) or in vivo (c) MIL-treated counterparts. Results are based on triplicate measurements of three biological replicates and are expressed as the mean FL2 fluorescence ± SE. *P < 0.05, ***P < 0.001

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