Fig. 3

Promastigote growth curves of wild-type parent (WT) and derived counterparts that were repeatedly exposed to MIL, either in vitro (a) or in vivo (b). No biologically significant differences could be observed for the in vitro MIL-exposed strain (a) whereas successive selection cycles in vivo resulted in parasites with a significantly increased in vitro promastigote growth (b). Results are expressed as the mean number of parasites in culture ± SE and are based on three independent replicates. *P < 0.05, **P < 0.01