Fig. 3
Analysis of putative positive colonies. a Mated bait-prey plasmids (transformed in yeast) showing blue colonies on DDO/X/A. b Two of the five blue clones patched out onto higher stringency QDO/X/A plates manifested blue patches. c Agarose gel electrophoresis of amplified PCR products for the inserts of putative positive prey plasmids. Lane M: DNA 5000 maker; Lanes 1 and 2: PCR amplification products of the inserts of the two putatively positive hits. d Mated prey plasmid and pGBKT7-VirB10 in Y2HGold cells cultivated on QDO/X/A plates showed blue colonies while mated prey plasmid and empty pGKBT7 showed no growth on QDO/X/A plates