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Fig. 4 | Parasites & Vectors

Fig. 4

From: Identification and evaluation of UL36 protein from Dermacentor silvarum salivary gland and its interaction with Anaplasma ovis VirB10

Fig. 4

Western blots of recombinant protein expression and GST pull-down results. a Expression of IPTG-induced recombinant protein His-VirB10 (approximately 57.7 kD). b Expression of IPTG-induced recombinant protein GST-UL36 (approximately 65.5 kD). c The input His-VirB10 and GST-tag mixture was eluded showing no binding between the His-VirB10 recombinant protein and GST-tag protein. d The fusion protein GST-UL36-His-VirB10 (approximately 123 kD) was obtained after GST pull-down assay using anti-His tag. e The input GST-UL36 and His-tag mixture was eluded showing no binding between the GST-UL36 recombinant protein and His-tag protein

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