Table 1 Primers and PCR amplification conditions
Primer name | Primer sequence (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|
VB10otF | AATTCCATATGATGTCAGACGAAACCAAGGATAAC | 58 | 1326 |
VB10otR | TCGACGTCGACCTACCTCCGCACCGCCTC | ||
V10-32F | CGCGGATCCATGTCAGACGAAACCAAGGATAA | 58 | 1340 |
V10-32R | AGAATGCGGCCGCCTACCTCCGCACCGCCTC | ||
S41-F | CGCGGATCCATGCGCCGCCACAAGGGT | 63.5 | 1139 |
S41-R | ATTTGCGGCCGCTCAGCGGCGGCGGATGAG |