Fig. 2From: New codon 198 β-tubulin polymorphisms in highly benzimidazole resistant Haemonchus contortus from goats in three different states in SudanAmino acid changes in codon 198 identified by Sanger sequencing. a–e Representative chromatograms. Vertical arrows mark polymorphisms in codon position one and/or two. Encoded amino acids are shown above the chromatograms with bold fonts indicated codons/amino acids proven to be present in Haemonchus contortus from South Darfur and normal fonts codons/amino acids potentially present. f Alignment of amino acids 191–206 with detected and potentially present amino acids in position 198 of isotype 1 β-tubulin gene of H. contortus using a sequence from a susceptible (S) isolate (GenBank: KX258905.1), a resistant isolate (GenBank: KX258927.1) and the sequences from the states South, Central and East Darfur, Sudan, with the different codon 198 polymorphisms. U, sequences obtained from samples collected from goats before albendazole treatment or from abattoirs with unknown resistance status. In the alignment, green indicates susceptible and red resistant variants at codon position 198. Variants known to confer benzimidazole resistance in fungi are shown in orange and variants with similar chemical properties as known resistant variants are coloured in yellowBack to article page