Fig. 4

Western blotting identification of rTsEla. a SDS-PAGE analysis of rTsEla. Lane M: protein marker; Lane 1: ML soluble proteins; Lane 2: ML ES proteins; Lane 3: purified rTsEla. b Western blotting revealed natural TsEla in soluble proteins of T. spiralis NBL (Lane 1), ML (Lane 2), IIL (Lane 3), 3d AW (Lane 4) and 6d AW (Lane 5) were probed by anti-rTsEla serum. c Western blotting showed that native TsEla in ES proteins of ML (Lane 1), IIL (Lane 2) and 3 d AW (Lane 3) were detected by anti-rTsEla serum. d Western blotting of rTsEla antigenicity. ML soluble protein (Lane 1), ML ES protein (Lane 2) and rTsEla (Lane3) were probed by T. spiralis-infected mouse sera as positive serum control; native TsEla in ML soluble (Lane 4) and ES protein (Lane 5), and rTsEla (Lane 6) were recognized by anti-rTsEla serum; ML soluble (Lane 7) and ES protein (Lane 8), and rTsEla (Lane 9) were not probed by uninfected mouse serum as negative serum control